Integrated analytical workflow for extracellular DNA (cfDNA) analysis for the assessment of testicular function in cases of severe male infertility (SPERMFREE)

Severe male infertility affects approximately 11% of men worldwide. Currently, to assess testicular function and determine whether sperm is being produced, doctors often use hormone tests, imaging studies, and sometimes invasive procedures, such as a testicular biopsy. These methods can be expensive, time-consuming, and do not always provide a clear answer, so patients often have to undergo multiple tests or even unnecessary surgeries.

Project SPERMFREE aims to develop a novel, non-invasive approach for assessing testicular function in cases of severe male infertility. The method is based on the analysis of extracellular DNA in ejaculate – various molecular markers are studied, and information on cellular activity and potential inflammatory processes is integrated using a computational model. This will allow doctors to more accurately determine whether sperm production is preserved, impaired, or not occurring at all, and consequently choose the most suitable treatment approach.

It is expected that such a diagnostic approach will reduce the number of unnecessary invasive procedures, shorten the diagnostic process, and help couples make informed family planning decisions more quickly. At the same time, it will reduce the costs of examination and treatment for both patients and the healthcare system, as well as lessen the emotional and time burden associated with lengthy infertility investigations.

– A novel, non-invasive diagnostic approach for the evaluation of male infertility

Fewer unnecessary invasive procedures and examinations, improving the patient experience and easing the work of healthcare professionals

– Faster and clearer diagnostic process in cases of severe infertility

– Reduced costs for patients and the healthcare system”